Application

Research CategoryEpigenetics & Nuclear Function

Detect p31comet using this mouse monoclonal antibody, Anti-p31comet Antibody, clone E29.19.14 validated for use in western blotting & IP.

Western Blotting Analysis: A representative lot from an independent laboratory detected p31comet in HeLa cells arrested in prometaphase (Mansfeld, J., et al. (2011). Nat Cell Biol. 13(10):1234-1243.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p31comet in HeLa cell lysates, which were untreated (asynchronous), arrested in nocodazole for 16 hr, or released in fresh medium for 2 hr (Varetti, G., et al. (2011). 44(5):710-720.).

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

p31comet is also called MAD2L1-binding protein (MAD2L1BP) and Caught by MAD2 protein (CMT2). p31comet silences the spindle checkpoint and allows mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. p31comet is expressed throughout the cell cycle, with levels increasing and then remaining constant until late mitosis after which they drop.

Immunogen

Recombinant protein corresponding to human p31comet.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected p31comet in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

35 kDa observed